Peptide Mapping with LC-HRMS and In Silico Tools for Evaluation of Potential Allergenicity of Commercially Available Whey Protein Hydrolysates

Hypoallergenic formulas containing hydrolyzed protein are intended for use by infants to prevent cow’s milk allergy. The degree of hydrolysis epitopic areas determines the residual allergenicity whey hydrolysates (WPHs). However, only amino-acid-based infant (IFs) considered entirely nonallergenic. aim this study was investigate four commercially available WPHs with different (H1–H4) potential allergenicity, applying LC-HRMS analysis peptides, and using in silico tools search immunoglobulin (IgE)-binding allergenic epitopes from Immune Epitope Database. Additionally, molecular weight distribution proteins peptides measured SE-UPLC. Based on peptide coverage peptide-length profiles, showed extents hydrolysis: extensively (H1 H2), partially (H3), slightly (H4). Altogether, numerous related 46 specific IgE-binding β-lactoglobulin α-lactalbumin were found all WPHs, regardless their extent hydrolysis. Sequence-based identification composition, an application tools, is a reliable approach discovering IFs.